Complete Adenovirus Production Service
ViraQuest Inc. offers complete adenovirus production services. Our team of scientists is here to assist you from the initial subcloning design to the final Quality Control (QC) on your new virus construct.
RAPAd® technology enables ViraQuest Inc. staff to produce your custom adenovirus particles in as little as 3 weeks. The majority of inserts can be subcloned in a single step in an easy to use shuttle plasmid. Our shuttle plasmids contain strong viral promoters for maximal gene expression or if tissue specific promoters are of interest, we can provide you with a promoterless shuttle to clone in your promoter of choice.
SUBCLONING – Our team can do the subcloning with the cDNA supplied by your lab or we can procure the cDNA for you. Our subcloning services include sequencing the 5’ and 3’ ends or the entire insert if PCR was used as part of the cloning strategy. If you prefer to carry out the subcloning, we can send our shuttle plasmids and provide technical assistance with the cloning strategy. The shuttles have an extensive MCS to facilitate directional subcloning of your insert. Please see the products page for more information on the shuttle plasmids.
RECOMBINATION – Once the shuttle has been confirmed by sequencing or functional analysis our team will recombine the shuttle plasmid with the appropriate backbone to generate virus particles suitable for amplification in our facility
AMPLIFICATION – The lysate containing the initial virus particles will be amplified prior to purification on CsCl gradients. If you have an adenovirus that you constructed in house or received from a collaborator, we can utilize your seed stock to amplify and purify the particles here at ViraQuest Inc.
PURIFICATION – All of our virus particles are purified over 2 rounds of CsCl gradients, dialyzed to remove the cesium and stored in one mL aliquots at 1X10^12 particles/mL. Our standard storage buffer contains TRIS buffer with sucrose as the cryoprotectant. These particles are suitable for direct injection for in vivo experiments or cell culture. If you have specific buffer requirements, we can formulate your virus prep in the storage buffer of your choice.
ULTRA CONCENTRATED PARTICLES – We can formulate the virus at higher concentrations when in vivo experiments require high virus load in low volumes. These preps of high concentration particles at 1X10^13 pts/mL are packaged in 100 uL aliquots.
PO Box 525, North Liberty, IA 52317
Phone: 319-665-4190 | Fax: 319-665-4191