We can make your novel adenovirus with a reporter gene in the E3 region of the backbone. Our method separates the expression cassettes by 20,000 bp and is more stable than expression cassettes placed in tandem in the E1 region. We can also engineer your second gene of choice into the E3 region. This eliminates the need for dual infection and guarantees that each cell receives both expression cassettes. You can be assured that each cell positive for the reporter construct is also expressing your shRNA or other gene product. Dual expressers can also be useful when delivering secreted gene products, heterodimers, or multivalent vaccines.
A single Adenovirus expressing nuclear-targeted B-gal and a secreted protein was injected into the mouse brain. Serial sections were stained for B-Gal (left panel) and for the secreted protein (right panel). Gene Therapy (2003) 10, 34-42
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