How long will it take?
Amplification of existing particles typically takes ~ 10 days. If we start with shuttle plasmids turn around time is generally 3 to 4 weeks.
How do I thaw and aliquot my samples?
We supply the virus in one mL aliquots and at 1X10^12 particles/mL. We recommend thawing the virus at room temperature and then aliquoting at least 50 uL in 0.5 mL screw cap tubes. Do not make aliquots in PCR tubes or eppendorf centrifuge tubes. We do not recommend diluting the virus and refreezing unless you use the cryo-protective buffer in the original formulation. Do not store or thaw the virus on ice, -20 C or 4 C.
If I run out of virus how long will it take to get more?
We try and always keep a stock of your purified particles in our freezers, so generally we can ship an aliquot of purified particles to you the next day. If we do not to have your materials in stock, we can generally amplify and purify a new prep for you in ~ 10 days.
Where do I find a list of premade viruses on your site?
Viruses that are widely used are available off the shelf, CsCl purified, contain 1X10^12 particles/mL and have been tested for RCA and PFU titered. These virus particles can be found on our products page. The vast majority of the viruses we make are custom constructs for our individual clients and these materials are held in confidence. These viruses may be available with permission from the originating PI. Please inquire for specific genes and we will determine how we can help.
What’s the big deal about RCA testing?
All viruses originating at ViraQuest Inc. are guaranteed to have non-detectable RCA.
RCA testing on amplified materials is particularly important, especially if you don’t know the history of the virus. About 10% of all the viruses sent to us from outside sources for amplification contain detectable Ad5 E1 sequences by PCR and are positive for viral plaques on non-permissive cell lines. The histories of these viruses generally follow this paradigm, ‘we got it from a lab that got it from some other lab. That’s all we know.’ If RCA is in the seed stock sent to us there will also be RCA in the purified materials. It is incumbent upon you to determine the RCA status of your seed stock prior to sending it to us for amplification. For amplified materials sent from outside sources we do not guarantee non-detectable RCA but will report the RCA test results to you.
Can I dilute the sample before injecting mice?
Yes. Common diluents include PBS and TBS. Once you dilute your sample do not refreeze the diluted samples as you have also diluted the cryoprotectants so the virus will likely be dead on the next thaw. Also, do not store or thaw the virus on ice, 4C or -20C. -80 or room temperature is best.
How do we send you our samples?
All virus samples should be sent via overnight carrier on dry ice. Please email the tracking number to firstname.lastname@example.org. DNA samples can be sent overnight in water or TE on cold packs. Please see our contacts pages for complete shipping address.
How do we pay for your services?
ViraQuest Inc. accepts Purchase Orders, MasterCard, Visa, and ACH transactions. ViraQuest Inc. is PCI compliant.
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PO Box 525, North Liberty, IA 52317
Phone: 319-665-4190 | Fax: 319-665-4191